AeroLab Reading room

Biology / AS level

Cell structure and microscopy

Eukaryotic cells compartmentalise reactions in membrane-bound organelles.

Learning objectives

The big idea

Eukaryotic cells compartmentalise reactions in membrane-bound organelles. Ribosomes synthesise proteins, rough endoplasmic reticulum supports processing and transport, and the Golgi apparatus modifies and packages products. Prokaryotes lack a membrane-bound nucleus but have DNA and ribosomes.

Electron microscopy resolves smaller structures than conventional light microscopy, but preparation can introduce artefacts. Magnification enlarges an image; resolution determines whether nearby details can be distinguished. Scale bars remain useful when images are resized.

Worked example

A 40 mm image represents a 20 µm structure. Find magnification.

Follow the solution

Convert 40 mm to 40000 µm, then divide by 20 µm: magnification is 2000.

Investigate and explain

Using the worked example as supplied data, predict the effect of changing one condition. Draw a labelled model, record your reasoning and identify a variable that would need controlling. No human, animal or microbial experiment is required.

No exact PhET model is claimed for this topic. Use diagrams and supplied-data reasoning instead.

Open the interactive investigation

Check your understanding

1. Which structure carries out translation?

  1. Golgi membrane alone
  2. Ribosome
  3. Cell wall
Check the answer and reasoning

Ribosome

Ribosomes assemble polypeptides using information carried by mRNA.

2. A 40 mm image represents a 20 µm structure. Find magnification.

  1. ×2
  2. ×800
  3. ×2000
Check the answer and reasoning

×2000

Convert 40 mm to 40000 µm, then divide by 20 µm: magnification is 2000.

3. Does enlarging a blurred image necessarily reveal new detail?

  1. No
  2. Yes
  3. Only on a large screen
Check the answer and reasoning

No

Magnification cannot recover features that the original imaging system failed to resolve.